Ensure data accuracy of your reader. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Cell proliferation assay MDA-MB-231 cells were seeded at a density. Open the Windows Task Manager, go to the Services tab, and click Open Services. Molecular Devices Corporation. TECHNICAL NOTE. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The average survival rates for cells exposed to dry. We would like to show you a description here but the site won’t allow us. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. 0 OD. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. Table 1 Conditions for biofilm formation on lids of 96-pin microtiter plates. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. S. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. Measurement of biofilm formation inhibitory activity. Select Start>Control panel>Device Manager. 1 mL. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. These models can no longer be repaired. We would like to show you a description here but the site won’t allow us. All animal experiments were performed in compliance with the relevant laws and. Sale. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. An aliquot of the samples dissolved in DMSO with 80 μL of phosphate buffer (pH 6. $199. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. $1,749. 99 shipping. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. The final mixtures were allowed to react at 25 °C in the dark for 5 min. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到With the VersaMax microplate reader, you will never have to buy another filter. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. 3, Molecular Devices). 0A Sunnyvale, CA Made in USA The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. +81-3-5282-5261 SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Bovine serum albumin was used as a reference. Shop on LabX Shop by Category Shop by Brand Shop by Application Shop by Reseller Sell on LabX Sell Your Equipment Advertise with LabX My AccountMolecular Devices Absorbance Microplate Reader VersaMax. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. StakMax Microplate Handling Stacker. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Table 1. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. SpectraMax®, VersaMax, VMax. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. Published Dec 16, 2020 | Updated May 19, 2023. VersaMax™ Tunable Microplate Reader, Molecular Devices. The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. TheMolecular Devices VersaMax Microplate Reader (ZB64) . Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. 2 and 648. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. The BMDM cells were plated into a 6-well culture plate at a density of 2. Microplate Reader Shelf. 5% NP-40) supplemented with protease and phosphatase inhibitor cocktail (GenDepot, Baker, TX, USA). Add to Cart. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. 2. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). Spectranet customer portalSpectraMax and VersaMax . 4. SpectraMax® Microplate Reader User Manual Download Page. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. Cell viability was analyzed using a cell counting kit assay (Dojindo). $1,195. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements allowing for selection of up to two wavelengths at a time. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 72 MB. Select up to two wavelengths. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. Find Fanuc, Molecular Devices, and GE for sale on Machinio. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). used. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 0, 0. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. A standard curve was made using the known concentrations of 4-nitrophenol (Sigma). ChE activity was normalized by protein concentration as determined by a Coomassie. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 72 MB. 1. Model: VERSAmax Tunable Microplate Reader. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). GABA concentrations in samples were calculated from calibration curves using the standard solutions. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). , Spectramax Gemini; Molecular Devices) Tissue culture plates (24- and 96-well) Vacuum suction pumpRelated products. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular Devices SoftMax Pro 5. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. BMG Labtech - THERMOstar. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. S. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. We would like to show you a description here but the site won’t allow us. 4. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. Molecular Devices Corporation. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. Used. The. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. The concentrations of anti-RSV G protein IgG antibodies in the samples were. Offer Ends In: 5 Days 05: 02: 03. 0%, 0-2. The absorbance of reaction mixtures was read at 475 nm using a microplate reader (VERSAMax, Molecular Devices) to determine the quantity of dopachrome produced. US HEADQUARTERS. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. 0 nm full width half maximum. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. , St. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. Data were analyzed with GraphPad Prism. Preparation of cytoplasmic and nuclear protein. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. The well plates were then washed with 1% acetic acid and dried. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel research methods to address key challenges of scaling complex 3D biological models. Filterless. 0 software (GraphPad). Spectranet customer portal. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. Find regional support. Microplate Reader Capability > UV - Vis Absorbance. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. Learn how to use SoftMax. The index was evaluated at 48 and 72 hours normalized to that at 24 hours. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 00 USD. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. StakMax Microplate Handling Stacker. The absorbance was read at 570 nm using a spectrophotometer (VersaMax, Molecular Devices, San Jose, CA, USA). Conventions Used in this Manual. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. All experiments were repeated three times. SoftMax Pro. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. S. The limit of detection for each assay was 156. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. (VersaMax Molecular Devices, Sunnyvale, CA). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. 5°C @ 37 °C W. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Molecular Devices SoftMax Pro 5. Unit price / per . ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. This document is provided to customers who have purchased Molecular Devices, Inc. After freeze-drying, the. The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Related products. 747. 2. After that, absornace were measured at 520 nm using using a microplate reader spectrophotometer VERSAmax (Molecular Devices). That's why our team is at your disposal the majority of time. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. DNA content in the supernatant (100 μl) was measured using a Quant-iT PicoGreen assay kit (Invitrogen) as per the manufacturer's instructions. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). absorbance readers. Cell cycle investigation. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. With a wavelength. TECHNICAL NOTE. Narrow bandwidths deliver increased accuracy and enhanced peak. E. VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. While the situation is vastly improving with numerous vaccine rollouts and over 6. 9. All samples were measured in triplicate. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Data were normalized to those of the PBS exposure control group. 9000-0756. Concentration was determined by BCA Protein Assay Kit (ref. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. 003 OD ±1. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Using a Spectradrop Micro-Volume Microplate (VersaMax, Molecular Devices, CA, USA), RNA was quantified, and 1 μg of total RNA was treated with DNase I (Thermo Scientific, Waltham, MA, USA) before. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. 파장 정확도 : < ±0. A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. Cellular imaging was performed on a Confocal microscope (Olympus IX 81 + FV1000, Shanghai, China) using an excitation wavelength of 380 nm. recombinant proteins, including monoclonal antibodies. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 4 (Molecular Devices cat. absorbance readers. After treatment with GA3The absorbance was measured with a microplate reader (VERSAmax, Molecular devices, San Jose, CA, USA) at 570 nm according to the manufacturer’s instructions. End-point IgG titers were established as the mean plus three standard deviations of. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. The system provides automation for up to 50For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. ¨We would like to show you a description here but the site won’t allow us. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. EUROPE. 4 Microplate Reader Software w WARRANTY. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Accession codes. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling System through the SoftMax Pro Software. 5°C @ 37°C Well-to. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Software (optional), such as Softmax Pro (Molecular Devices). SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. The absorbance was measured at 570 nm and. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. from Molecular Devices Corporation. SpectraMax® i3 Multi-Mode Detection Platform. . Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The percentages of inhibition of cell viability were calculated as the ratio between the absorbance of the compound-treated cells and cells with only the vehicle. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Access all the resources, tools and information you need to stay productive. 2. Total. The sections were stained with hematoxylin and eosin (H&E) and. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). 00 USD. SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. 4. The SpectraTest® ABS1 Absorbance. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. Cell viability was assessed using the WST-1 assay. g. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. Sale Sold out. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. , Sunnyvale, CA). Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 00. $1,195. The in vitro differentiation of BMDMs. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). 4 Microplate Reader Software w WARRANTY. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). The cellular viability. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including: vaccines. Tunable, filterless flexibility The. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. It was verified that absorbance values were proportional to the amount of a bound peroxidase-labeled antibody. And the VERSAmax reader is robotics-compatible. Add to Cart. A solution of 10 millimolar L-DOPA (40 µL) was added to each sample and the blank. Thus, the molecular mechanism promoting hepatic lipid accumulation in obesity is of biomedical and public health interest. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Used. Windows Home Editions are not supported. The detection limit for rivaroxaban in this assay was 10 ng/mL. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. All analyses were performed three times, and the mean values were used for further calculations. from Molecular Devices Corporation. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. StakMax Microplate Handling System. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. 3860 N First Street San Jose,CA 95134 USA +1 408. 키네틱 읽기 시간 : 최소 9초 간격. Lipid accumulation was assessed by ORO staining. 00. 00 Sale price $ 0. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. 17. This technical note is designed to provide information . While the cell covers about. 00 Sale price $ 0. 00. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. [Google. The absorbance of the treated and untreated samples at 450 nm was then measured on a multi-well microplate reader (VersaMax, Molecular devices, San Jose, CA, USA). The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Also see: SoftMax® Pro Microplate Software User Manual Download Page. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. 1700 +1 800. Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. . The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. . S. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. Molecular Devices, LLC. Cell proliferation assay MDA-MB-231 cells were seeded at a density. Glossary of Terms. Louis, USA) was used to. microtiter plate reader (VERSAmax, Molecular Devices). 03 billion doses of. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. , USA) at 490 nm. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. All. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). This. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. (Molecular Research Center, USA) according to the manufacturer’s. Never order or replace filters to optimize an assay again! Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. ® Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Get pricing and purchase lab equipment and.